Human lipoproteins have divergent neutralizing effects on E. coli LPS, N. meningitidis LPS, and complete Gram-negative bacteria

Human lipoproteins have divergent neutralizing effects on E. coli LPS, N. meningitidis LPS, and complete Gram-negative bacteria.

The use of lipoproteins has been suggested as a treatment for Gram-negative sepsis because they inhibit lipopolysaccharide (LPS)-mediated cytokine production. However, little is known about the neutralizing effects of lipoproteins on cytokine production by meningococcal LPS or whole Gram-negative bacteria. We assessed the neutralizing effect of LDLs, HDLs, and VLDLs on LPS- or whole bacteria-in...

Inflammatory Response and Gram-Negative Bacteria-Induced Monocytic Thrombomodulin Triggers LPS-

Gram-negative trimeric porins have specific LPS binding sites that are essential for porin biogenesis.

The outer membrane (OM) of gram-negative bacteria is an unusual asymmetric bilayer with an external monolayer of lipopolysaccharide (LPS) and an inner layer of phospholipids. The LPS layer is rigid and stabilized by divalent cation cross-links between phosphate groups on the core oligosaccharide regions. This means that the OM is robust and highly impermeable to toxins and antibiotics. During t...

A Molecular Mechanism for Lps Protection of Gram-negative Bacteria from Antimicrobial Peptides

A MOLECULAR MECHANISM FOR LPS PROTECTION OF GRAM-NEGATIVE BACTERIA FROM ANTIMICROBIAL PEPTIDES Niv Papo and Yechiel Shai* Department of Biological Chemistry. The Weizmann Institute of Science Rehovot, 76100 Israel. Running title: Bacterial Susceptibility and LPS Permeability of Peptide Diastereomers Addresse correspondence to Yechiel Shai, Department of Biological Chemistry, The Weizmann Instit...

Complement activation induced by purified Neisseria meningitidis lipopolysaccharide (LPS), outer membrane vesicles, whole bacteria, and an LPS-free mutant.

Complement activation is closely associated with plasma endotoxin levels in patients with meningococcal infections. This study assessed complement activation induced by purified Neisseria meningitidis lipopolysaccharide (Nm-LPS), native outer membrane vesicles (nOMVs), LPS-depleted outer membrane vesicles (dOMVs), wild-type meningococci, and an LPS-free mutant (lpxA(-)) from the same strain (44...